The recBC Deoxyribonuclease of Escherichia coli K-12
نویسندگان
چکیده
منابع مشابه
Modulation of the action of the recBC enzyme of Escherichia coli K-12 by Ca2+.
The various activities of the recBC enzyme of Escherichia coli are differentially sensitive to reaction conditions. Although spermidine up to 50 mM and putrestine up to 40 mM in the presence of Mg2+ produce no significant alteration in the activities of the enzyme, low concentrations of Ca”’ have a dramatic effect. In the presence of 1 mM Ca2+, 1 mM Mg’+, and 5 mM ATP, the DNA-dependent ATPase ...
متن کاملPurification and properties of the recBC DNase of Escherichia coli K-12.
The deoxyribonuclease controlled by the recB and recC loci of Escherichia coli K-12 has been purified to near homogeneity. It is composed of two nonidentical polypeptide chains with a combined molecular weight of approximately 270,000. In the presence of ATP the enzyme hydrolyzes linear duplex or single stranded DNA in an exonucleolgtic manner to 5’-phosphoryl-terminated oligonucleotides cf an ...
متن کاملGeneral recombination in Escherichia coli K-12: in vivo role of RecBC enzyme.
Heterozygous lacZ- merodiploids of Escherichia coli K-12 have been used to study the role of the RecBC enzyme in general recombination. The transcribable intermediate assay detects the product of early steps in recombination without requiring the formation of viable recombinant colonies. Recombination is initiated by infection with lambda precA+. We have found that transcribable intermediate fo...
متن کاملEffect of transcription on RecBC- and RecF-mediated recombination within the tryptophan operon of Escherichia coli K-12.
Recombination between tryptophan gene mutations within the trp operon was determined among transductants for an outside linked cysB marker under conditions of repression and derepression. These studies, carried out with recipient strains utilizing the RecBC or RecF pathway, or a combination of these pathways of recombination, demonstrate that transcription of trp genes as regulated by the trp r...
متن کاملThe mechanism of degradation of duplex deoxyribonucleic acid by the recBC enzyme of Escherichia coli K-12.
Reaction intermediates formed during the degradation of duplex DNA by the recBC DNase have been characterized by sedimentation velocity, isopyknic centrifugation, electron microscopy, and susceptibility to various deoxyribonucleases which are specific for single-stranded DNA. It has been concluded that the intermediates are of two classes: (a) duplex molecules with single-stranded tails of seve...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43644-2